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KMID : 0379519990150030289
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한국독성학회지
1999 Volume.15 No. 3 p.289 ~ p.295
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The Effects of Sodium Arsenite on Bone Marrow Cells Stimulated by GM-CSF
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Han Seong-Su
Chung Hye-Joo
Park Jae-Hyun
Cho Dae-Hyun
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Abstract
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The effects of arsenic on the proliferation and differentiation of bone marrow cells were studied by treatment of bone marrow cells with sodium arsenite and/or granulocyte/macrophage-colony stimulating factor (GM-CSF). GM-CSF stimulated the bone marrow cells, and the maximum growth rate of bone marrow cells following exposure to GM-CSF for 1, 3, 5 and 7 days was 133, 275, 400 and 208% of the control respectively. When bone marrow cells were treated with sodium arsenite ranging from 0.01 to 50 μM for 1, 3, 5 or 7 days, the growth of bone marrow cells was inhibited by dose- and time-dependent manner at the concentration higher than 1 μM, and the mean IC50 value was 6.2, 7.5, 9.0 and 7.7 μM, respectively. Apoptosis was also observed in all groups treated with 10 μM sodium arsenite regardless of duration of treatment. By exposure of bone marrow cells to sodium arsenite concomitant with 10 μM GM-CSF for 1, 3, 5 and 7 days, IC50 value in each group was shifted to the higher value. These results indicate that fast proliferating bone marrow cells by stimulation with GM-CSF are likely to be less vulnerable to inhibiting effects of arsenic on the cell growth, and apoptosis induced by high concentration of sodium arsenite could be suggested as one of the mechanism for arsenic in treatment of leukemia.
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KEYWORD
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Sodium arsenite(NaAs)2), GM-CSF, Bone marrow cell culture, Proliferation, Apoptosis
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